Doublejellyroll capsid protein, giant icosahedral dna viruses. Solution structure of dengue virus capsid protein reveals another fold lixin ma, christopher t. Ndvi cdr is a global climatological data record of vegetation. Dengue virus denv is a positivesense, singlestranded rna virus of the family flaviviridae.
Dengue virus ns2b protein targets cgas for degradation and. The disease is caused by the dengue virus, a flavivirus that uses mosquitos aedes sp. Here, we report on experiments of laboratory infections of ae. Poliovirus, the causative agent of polio also known as poliomyelitis, is a serotype of the.
The virus core, within a lipid bilayer, has a lessordered structure than the external, icosahedral scaffold of 90 glycoprotein e dimers. A virus spread through aedes species mosquito bites. Virus pathogen database and analysis resource vipr. Most of these images are 1200 x 784 pixels and saved qith quality 12 maximum from photoshop. The overall fit of the 90 tbev e dimer structures to the cryoem map of dengue virus gives a satisfying, closepacking arrangement figures 3b and 3d. This file is licensed under the creative commons attributionshare alike 3. Clinical findings chikungunya and dengue clinical and laboratory features of chikungunya virus infections compared with dengue virus infections treatment and clinical management. During the last few decades, the global incidence of dengue virus denv has increased dramatically, and it is now endemic in more than 100 countries. A risk to anyone traveling to a tropical or subtropical region of the world where dengue virus is found. In many cities, rapid urbanization has resulted in densely packed human and mosquito populations and in an increased. Due to economic and touristic importance, the city receives a large daily influx of people from different regions, a situation that may increase the risk of entry and. Dengue virus denv is the causative agent of dengue fever, the most important arboviral disease of humans.
Coevolution at the molecular level is an established phenomenon exhibited by organisms in order to optimize their physiological performance and serve as an effective determinant of fitness 1, 2. West nile virus wnv is a singlestranded rna virus that causes west nile fever. The raw fastq files for each virus sample were filtered. This outbreak was caused by only one serotype, den1 virus, and it was declared controlled march 12, 20. Though dengue normally causes a selflimiting infection, some patients may develop a lifethreatening illness, dengue hemorrhagic fever dhfdengue shock syndrome dss. Mapping of chikungunya virus interactions with host proteins identi. Knowledge, attitude and practices of vectorborne disease. Wolbachia wolbachia is not usually found in the aedes aegypti mosquito, the primary species responsible for transmitting harmful diseases such as zika, dengue and chikungunya. Vector competence of aedes japonicus for chikungunya and. Vector competence of populations of aedes aegypti from three distinct cities in kenya for chikungunya virus sheila b. Dengue virus is a small virus that carries a single strand of rna as its genome. Progress in the identification of dengue virus entry.
Dengue has been recognized as one of the most important vectorborne emerging infectious diseases globally. Viral culture in the first 3 days of illness may be utilized. Neotropical bats that cohabit with humans function as. The images also appear when selecting from individual virus names. Deep sequencing and rna structure analysis, together with fitness evaluation, revealed a process of host specialization of. Dengue viruses are spread by mosquitos and can cause a deadly hemorrhagic fever or a milder illness. View dengue control project research papers on academia. The international committee on taxonomy of viruses ictv has the task of developing. Agha 1, edith chepkorir, francis mulwa1, caroline tigoi1, samwel arum1, milehna m. Coevolution refers to synchronized changes that organisms or proteins undergo and can occur with respect to pathogenhost, as well as within the organism 4, 5, often revealing functional. Zhao y, soh ts, zheng j, chan kwk, phoo ww, lee cc, et al. Virus structure, classification and nomenclature, viral replication and multiplication, virus induced changes and pathology, mechanisms of virus infection, virus immunology, bacteriophages and parvoviruses.
Dengue fever, a reemerging disease, is putting nearly 2. Mature viral protein products resulting from protease and selfcleavage of the polyprotein are displayed according to their location in the genome and the polyprotein. Assembly of dsrna bacterial viruses the role of lipid rafts in virus replication structural transformations of viral fusion proteins the heads of phage hk97. Feat provides insights into west nile, yellow fever and other flaviviruses. Guarido2, peris ambala3, betty chelangat4, joel lutomiah4, david p. The dengue virus structure has sets of three, nearly parallel dimers.
The virus core, within a lipid bilayer, has a lessordered structure than the external, icosahedral scaffold of 90. A crystal structure of the dengue virus ns5 protein reveals a novel interdomain interface essential for protein flexibility and virus replication. Welcome to vectorbases population biology popbio resource. Decoration options let you color tree leaves by metadata. Effective and novel mechanisms to block chikungunya virus identified. Chikungunya testing is done at the cdc, a few state health departments, and one commercial laboratory. Bunyaviridae, flaviviridae and togaviridae that use arthropods such as mosquitoes, flies and ticks as vectors for transmission between different hosts.
Aedes mosquitoes also spread dengue and zika viruses. The disease has several clinical manifestations, from the mild coldlike illness to the. Environmental data sources for the dengue project 1. Each serotype is divided to several different genotypes, such as there are 5 genotypes in dengue virus serotype 3 denv3, in which genotype ii was confirmed to be the main etiology cause the big dengue feverdengue hemorrhagic fever outbreak in vietnam 1998. The structure of mature dengue virus particles was determined to a resolution of 9.
The protein shell is made of two structural proteins. A crystal structure of the dengue virus polymerase reveals that linker residues contribute to protein stability. Global risk outbreaks have occurred in parts of africa, europe. Author summary dengue virus denv is a major arthropodborne human pathogen, infecting more than 400 million individuals annually worldwide.
The four denv serotypes denv1, 2, 3, and 4 are primarily transmitted between humans by the mosquito aedes aegypti, with aedes albopictus as a secondary vector. These data are available for each city on a weekly basis from 1981present. In this paper, we discuss recent advances in our knowledge of the dengue virus life cycle based on new structural data of the virus and its proteins. Dengue virus nonstructural protein 1 modulates infectious.
To study the mosquitohuman adaptation cycle, we examined changes in rna structures of the dengue virus genome during host adaptation. Virus structure and assembly, volume 64 1st edition elsevier. The first structure of a flavivirus has been determined by using a combination of cryoelectron microscopy and fitting of the known structure of glycoprotein e into the electron density map. Solution structure of dengue virus capsid protein reveals.
Poliovirus, rabies virus the type of disease murine leukemia virus geographic locations sendai virus, coxsackie virus their discovers epsteinbarr virus how they were originally thought to be contracted dengue virus evil spirit, influenza virus the influence of bad air combinations of the above. Vipr uses phyml guindon and gascuel, 2003 and offers multiple evolutionary models to infer phylogenies. Rebeca ricohesses laboratory is studying the structural rna andor protein and transmission replication in aedes aegypti characteristics of dengue type 2 and type 3 virus variants that have produced dengue hemorrhagic fever in humans throughout the world. The envelope of the virus is formed when the virus is exiting the cell via budding, and the infectivity of these viruses is mostly dependent on the envelope. This is a pdf file of an unedited manuscript that has been.
Opening doors to the possibility of developing new vaccines and antiviral agents to fight a host of insectborne diseases, scientists have, for the first time, determined the structure of a family of viruses known as the flaviviruses. A crystal structure of the dengue virus ns5 polymerase. Arboviruses arthropodborne viruses are an ecological group of viruses from different families e. The structural biology of dengue virus request pdf. The other three viruses, den2, den3, and den4, are closely related to den1, but are not recognized as the same virus by. Dengue virus rna structure specialization facilitates host. The number of infections and the geographic extension of dengue fever infection have increased in the past decade. Dengue virus 2d black arrowhead, 3d red particle buds white arrows into the endoplasmic reticulum opposite to virusinduced vesicles 3d yellow structure. A threedimensional image reconstruction shows that the virion has a wellorganized outer protein shell, a lipid bilayer membrane, and a lesswelldefined inner nucleocapsid core. A presentation on dengue virus structure, how the virus attacks and spreads in the body, role of heterocyclic drugs in inhibiting the virus and our experiments slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. We report the structure of dengue virus type 2 s1 strain, a vaccine candidate derived from the pr159 isolate hahn et al.
Three of these are structural proteins that form the coat of the virus and deliver the rna to target cells, and seven of them are nonstructural proteins that orchestrate the production of new viruses once the virus gets inside the cell. Wolbachia is natural bacteria present in up to 60% of. Lab diagnosis by testing of serum or plasma for virus, viral nucleic acid or virus specific igm. In recent decades the aedes mosquitoes carrying chikungunya have spread to the european region. Dengue virus 1 den1 was first discovered in japan in the 1940s. This lesson covers the differences between the types dengue viruses and how they cause disease. Species of dead birds in which west nile virus has been detected, united states, 19992016 pdf. There are four dengue virus serotypes, based on molecular analysis. The ns5 protein from dengue virus comprises a methyltransferase and a polymerase domain connected by a linker region.
Effective and novel mechanisms to block chikungunya virus. Dengue virus enters a host cell when the viral envelope glycoprotein, e, binds to a receptor and responds by conformational rearrangement to the reduced ph of an endosome. Chikungunya virus antibodies normally develop toward the end of the first week of. Thus, we are unable to identify the family of sequences coding for similar protein structures.
Amongst the denv proteins, nonstructural protein 1 ns1 is one of the most enigmatic, being required for rna replication, but also secreted from infected cells to. You may do so in any reasonable manner, but not in. Strains within the same species shared 70% of their bands in common. Composition and threedimensional architecture of the. Between 50 and 100 million cases are reported annually, and up to 500,000 people are admitted to hospitals with dengue hemorrhagic fever and dengue shock syndrome gubler, 2002, halstead, 1988, halstead, 2007.
Genomic characterization and epidemiology of 2019 novel. Symptoms are fever, joint and muscle pain, headache, nausea, fatigue and rash. Each year, an estimated 390 million people are infected. The reason why dhfdss occurs in certain individuals is unclear. A risk to anyone traveling to a region of the world where chikungunya virus is found. Differential proteomic analysis of virusenriched fractions obtained from plasma pools of patients with dengue fever or severe dengue romain fragnoud1, marie flamand2, frederic reynier3, philippe buchy4, vasna duong4, alexandre pachot1, glaucia paranhosbaccala5 and frederic bedin6 abstract. The structure of a large fragment of the ectodomain of e1 protein of sindbis virus has been determined and was suggested to be homologous to the e glycoprotein found in flaviviruses 25. In the portuguese island of madeira, the rst outbreak of dengue fever occurred between october 2012 and february 20. Linker residues enhance polymerase activity and thermostability.
The structure of the virus was first elucidated using xray diffraction by a team at birkbeck. Structure of the dengue virus envelope protein after. These sets associate to form a herringbone configuration on the viral surface. Vector competence of populations of aedes aegypti from. Specifically, we focus on the structure of the premembrane protein, prm and its role in virus assembly, the first fulllength structure of a multi. This virus structure is a conventional icosahedral or helical structure that is surrounded by a lipid bilayer membrane, meaning the virus is encased or enveloped. Analysis of coevolution in nonstructural proteins of. Coexistence of two dengue virus serotypes and forecasting.
954 820 1627 384 1184 1167 1549 98 10 1480 1435 59 1174 718 1593 280 280 699 825 273 1052 913 250 758 494 789 158 578 257 951 1617 82 628 1476 781 1506 1626 487 210 1012 645 530 938 643 239 433 50 1112 1401